Transient gene expression from mammalian cells – a newchapter in animal cell technology?A report on the First European Technology Workshop on:Current Status and Trends in Mammalian...

Por Wurm, Florian, Bernard, Alain
Publicado em 2001

Calcium-phosphate mediated DNA transfer into HEK-293 cells in suspension: control of physicochemical parameters allows transfection in stirred media. Transfection and protein expre...

Por Jordan, Martin, Köhne, Christiane, Wurm, Florian M.
Publicado em 1998

Extraction of plasmid DNA using reactor scale alkaline lysis and selective precipitation for scalable transient transfection

Por Wright, Jason L., Jordan, Martin, Wurm, Florian M.
Publicado em 2001

Multivalent Ions as Reactive Crosslinkers for Biopolymers—A Review

Por Wurm, Florian, Rietzler, Barbara, Pham, Tung, Bechtold, Thomas
Publicado no Molecules (2020)

S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi

Por Grosjean, Frédéric, Batard, Pascal, Jordan, Martin, Wurm, Florian M.
Publicado em 2002

Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells

Por Davami, Fatemeh, Baldi, Lucia, Rajendra, Yashas, M. Wurm, Florian
Publicado em 2014

Study of the improved Sf9 transient gene expression process

Por Shen, Xiao, Hacker, David L, Baldi, Lucia, Wurm, Florian M
Publicado em 2013

Calcium phosphate transfection optimization for serum-free suspension culture

Por Girard, Philippe, Porte, Luc, Berta, Temugin, Jordan, Martin, Wurm, Florian M.
Publicado em 2001

Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin(®) bioreactors

Por Pereira, João, Rajendra, Yashas, Baldi, Lucia, Hacker, David L, Wurm, Florian M
Publicado em 2011

Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells

Por Rajendra, Yashas, Kiseljak, Divor, Baldi, Lucia, Hacker, David L, Wurm, Florian M
Publicado em 2011

Transposon mediated co-integration and co-expression of transgenes in CHO-DG44 cells

Por Balasubramanian, Sowmya, Matasci, Mattia, Baldi, Lucia, Hacker, David L, Wurm, Florian M
Publicado em 2011

k(L)a as a predictor for probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors

Por Tissot, Stéphanie, Monteil, Dominique T, Baldi, Lucia, Hacker, David L, Wurm, Florian M
Publicado em 2011

Transient transfection of insect Sf-9 cells in TubeSpin(®) bioreactor 50 tubes

Por Shen, Xiao, Michel, Patrik O, Xie, Qiuling, Hacker, David L, Wurm, Florian M
Publicado em 2011

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

Por Backliwal, Gaurav, Hildinger, Markus, Chenuet, Sebastien, Wulhfard, Sarah, De Jesus, Maria, Wurm, Florian M.
Publicado em 2008

The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells

Por Kiseljak, Divor, Rajendra, Yashas, Manoli, Sagar S, Baldi, Lucia, Hacker, David L, Wurm, Florian M
Publicado em 2011

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells

Por Hacker, David L., Kiseljak, Divor, Rajendra, Yashas, Thurnheer, Sarah, Baldi, Lucia, Wurm, Florian M.
Publicado no Protein Expr Purif (2013)

Rapid recombinant protein production from pools of transposon-generated CHO cells

Por Matasci, Mattia, Bachmann, Virginie, Baldi, Lucia, Hacker, David L, De Jesus, Maria, Wurm, Florian M
Publicado em 2011

CHO cell lines generated by PiggyBac transposition

Por Matasci, Mattia, Bachmann, Virginie, Baldi, Lucia, Hacker, David L, De Jesus, Maria, Wurm, Florian M
Publicado em 2011

100-liter transient transfection

Por Girard, Philippe, Derouazi, Madiha, Baumgartner, Gwendoline, Bourgeois, Michaela, Jordan, Martin, Jacko, Barbara, Wurm, Florian M.
Publicado em 2002

The optimization of a rapid low-cost alternative of large-scale medium sterilization

Por Monteil, Dominique T, Bürki, Cédric A, Baldi, Lucia, Hacker, David L, de Jesus, Maria, Wurm, Florian M
Publicado em 2013