Engineering Plant Secondary Metabolism in Microbial Systems

per Pyne, Michael E., Narcross, Lauren, Martin, Vincent J.J.
Publicat a Plant Physiol (2019)

Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum

per Pyne, Michael E, Moo-Young, Murray, Chung, Duane A, Chou, C Perry
Publicat 2013

Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme

per Pyne, Michael E, Moo-Young, Murray, Chung, Duane A, Chou, C Perry
Publicat a Biotechnol Biofuels (2014)

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

per Pyne, Michael E., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Publicat a Appl Environ Microbiol (2015)

Genome-directed analysis of prophage excision, host defence systems, and central fermentative metabolism in Clostridium pasteurianum

per Pyne, Michael E., Liu, Xuejia, Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Publicat a Sci Rep (2016)

Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium

per Pyne, Michael E., Bruder, Mark R., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Publicat a Sci Rep (2016)

Extending CRISPR-Cas9 Technology from Genome Editing to Transcriptional Engineering in the Genus Clostridium

per Bruder, Mark R., Pyne, Michael E., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Publicat a Appl Environ Microbiol (2016)

Using the Endogenous CRISPR-Cas System of Heliobacterium modesticaldum To Delete the Photochemical Reaction Center Core Subunit Gene

per Baker, Patricia L., Orf, Gregory S., Kevershan, Kimberly, Pyne, Michael E., Bicer, Taner, Redding, Kevin E.
Publicat a Appl Environ Microbiol (2019)

Improved Draft Genome Sequence of Clostridium pasteurianum Strain ATCC 6013 (DSM 525) Using a Hybrid Next-Generation Sequencing Approach

per Pyne, Michael E., Utturkar, Sagar, Brown, Steven D., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Publicat 2014

An Engineered Aro1 Protein Degradation Approach for Increased cis,cis-Muconic Acid Biosynthesis in Saccharomyces cerevisiae

per Pyne, Michael E., Narcross, Lauren, Melgar, Mindy, Kevvai, Kaspar, Mookerjee, Shoham, Leite, Gustavo B., Martin, Vincent J. J.
Publicat a Appl Environ Microbiol (2018)

A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids

per Pyne, Michael E., Kevvai, Kaspar, Grewal, Parbir S., Narcross, Lauren, Choi, Brian, Bourgeois, Leanne, Dueber, John E., Martin, Vincent J. J.
Publicat a Nat Commun (2020)

Disruption of the Reductive 1,3-Propanediol Pathway Triggers Production of 1,2-Propanediol for Sustained Glycerol Fermentation by Clostridium pasteurianum

per Pyne, Michael E., Sokolenko, Stanislav, Liu, Xuejia, Srirangan, Kajan, Bruder, Mark R., Aucoin, Marc G., Moo-Young, Murray, Chung, Duane A., Chou, C. Perry
Publicat a Appl Environ Microbiol (2016)