Purification and Characterization of Borrelia burgdorferi from Feeding Nymphal Ticks (Ixodes scapularis)

Here we describe a protocol for purifying Borrelia burgdorferi from feeding ticks by velocity centrifugation and Percoll density gradient centrifugation. The purified spirochetes were motile and 10- to 20-fold purer than the bacteria in crude tick homogenates. The purified bacteria were present in s...

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Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	Rathinavelu, Sivaprakash, de Silva, Aravinda M.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Molecular Pathogenesis
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC98328/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11349010/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/IAI.69.6.3536-3541.2001
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