Purification and Characterization of Borrelia burgdorferi from Feeding Nymphal Ticks (Ixodes scapularis)
Here we describe a protocol for purifying Borrelia burgdorferi from feeding ticks by velocity centrifugation and Percoll density gradient centrifugation. The purified spirochetes were motile and 10- to 20-fold purer than the bacteria in crude tick homogenates. The purified bacteria were present in s...
Gardado en:
| Publicado en: | Infect Immun |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
2001
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC98328/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11349010/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/IAI.69.6.3536-3541.2001 |
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