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Purification and Characterization of Borrelia burgdorferi from Feeding Nymphal Ticks (Ixodes scapularis)
Here we describe a protocol for purifying Borrelia burgdorferi from feeding ticks by velocity centrifugation and Percoll density gradient centrifugation. The purified spirochetes were motile and 10- to 20-fold purer than the bacteria in crude tick homogenates. The purified bacteria were present in s...
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| Glavni autori: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology
2001
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC98328/ https://ncbi.nlm.nih.gov/pubmed/11349010 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.69.6.3536-3541.2001 |
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