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Restriction enzymes increase efficiencies of illegitimate DNA integration but decrease homologous integration in mammalian cells

Mammalian cells repair DNA double-strand breaks by illegitimate end-joining or by homologous recombination. We investigated the effects of restriction enzymes on illegitimate and homologous DNA integration in mammalian cells. A plasmid containing the neo(R) expression cassette, which confers G418 re...

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Bibliografske podrobnosti
Main Authors: Manivasakam, Palaniyandi, Aubrecht, Jiri, Sidhom, Samy, Schiestl, Robert H.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2001
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC96699/
https://ncbi.nlm.nih.gov/pubmed/11726692
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