Use of Formalin-Fixed, Propidium Iodide-Stained Human Leukocytes as a Standard for Enumerating CD4(+) T Lymphocytes in a Single-Platform Assay

A new, inexpensive method is described that enables lymphocytes to be enumerated very precisely. Normal leukocytes were simultaneously stained and fixed with a propidium iodide-paraformaldehyde solution. The preparation obtained (CellBeads) was used as an internal standard for cell enumeration by fl...

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Bibliographische Detailangaben
Hauptverfasser: Harrison, G. M., Bennett, A. J., Moody, M., Read, G. F., Williams, P. E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2001
Schlagworte:
Cellular Immunology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC96069/
https://ncbi.nlm.nih.gov/pubmed/11238228
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CDLI.8.2.397-401.2001
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