Evaluation of Recombinant Dense Granule Antigen 7 (GRA7) of Toxoplasma gondii for Detection of Immunoglobulin G Antibodies and Analysis of a Major Antigenic Domain
Dense granule protein 7 (GRA7) of Toxoplasma gondii was expressed in Escherichia coli as a fusion protein. The leader peptide contained a 25-amino-acid mouse tumor necrosis factor fragment and six histidyl residues. After purification by metal chelate affinity chromatography, the antigen was evaluat...
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| Published in: | Clin Diagn Lab Immunol |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1999
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC95655/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9874659/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/cdli.6.1.24-29.1999 |
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