Use of Transposon Tn5367 Mutagenesis and a Nitroimidazopyran-Based Selection System To Demonstrate a Requirement for fbiA and fbiB in Coenzyme F(420) Biosynthesis by Mycobacterium bovis BCG

Three transposon Tn5367 mutagenesis vectors (phAE94, pPR28, and pPR29) were used to create a collection of insertion mutants of Mycobacterium bovis strain BCG. A strategy to select for transposon-generated mutants that cannot make coenzyme F(420) was developed using the nitroimidazopyran-based antit...

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Detalhes bibliográficos
Main Authors: Choi, Kwang-Pil, Bair, Thomas B., Bae, Young-Min, Daniels, Lacy
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC95553/
https://ncbi.nlm.nih.gov/pubmed/11717263
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.24.7058-7066.2001
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