Insertional Mutagenesis in the n-Alkane-Assimilating Yeast Yarrowia lipolytica: Generation of Tagged Mutations in Genes Involved in Hydrophobic Substrate Utilization

Tagged mutants affected in the degradation of hydrophobic compounds (HC) were generated by insertion of a zeta-URA3 mutagenesis cassette (MTC) into the genome of a zeta-free and ura3 deletion-containing strain of Yarrowia lipolytica. MTC integration occurred predominantly at random by nonhomologous...

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Autores principales: Mauersberger, Stephan, Wang, Hui-Jie, Gaillardin, Claude, Barth, Gerold, Nicaud, Jean-Marc
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2001
Materias:
Eukaryotic Cells
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC95386/
https://ncbi.nlm.nih.gov/pubmed/11489863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.17.5102-5109.2001
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