Insertional Mutagenesis in the n-Alkane-Assimilating Yeast Yarrowia lipolytica: Generation of Tagged Mutations in Genes Involved in Hydrophobic Substrate Utilization

Tagged mutants affected in the degradation of hydrophobic compounds (HC) were generated by insertion of a zeta-URA3 mutagenesis cassette (MTC) into the genome of a zeta-free and ura3 deletion-containing strain of Yarrowia lipolytica. MTC integration occurred predominantly at random by nonhomologous...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Mauersberger, Stephan, Wang, Hui-Jie, Gaillardin, Claude, Barth, Gerold, Nicaud, Jean-Marc
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Eukaryotic Cells
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC95386/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11489863/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.183.17.5102-5109.2001
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