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Biological and Biochemical Characterization of Variant A Subunits of Cholera Toxin Constructed by Site-Directed Mutagenesis
Cholera toxin (CT) is the prototype for the Vibrio cholerae-Escherichia coli family of heat-labile enterotoxins having an AB5 structure. By substituting amino acids in the enzymatic A subunit that are highly conserved in all members of this family, we constructed 23 variants of CT that exhibited dec...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2001
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC95286/ https://ncbi.nlm.nih.gov/pubmed/11395467 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.13.4024-4032.2001 |
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