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Biological and Biochemical Characterization of Variant A Subunits of Cholera Toxin Constructed by Site-Directed Mutagenesis

Cholera toxin (CT) is the prototype for the Vibrio cholerae-Escherichia coli family of heat-labile enterotoxins having an AB5 structure. By substituting amino acids in the enzymatic A subunit that are highly conserved in all members of this family, we constructed 23 variants of CT that exhibited dec...

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Detalhes bibliográficos
Main Authors: Jobling, Michael G., Holmes, Randall K.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC95286/
https://ncbi.nlm.nih.gov/pubmed/11395467
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.13.4024-4032.2001
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