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Engineering a Homo-Ethanol Pathway in Escherichia coli: Increased Glycolytic Flux and Levels of Expression of Glycolytic Genes during Xylose Fermentation

Replacement of the native fermentation pathway in Escherichia coli B with a homo-ethanol pathway from Zymomonas mobilis (pdc and adhB genes) resulted in a 30 to 50% increase in growth rate and glycolytic flux during the anaerobic fermentation of xylose. Gene array analysis was used as a tool to inve...

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Main Authors: Tao, Han, Gonzalez, Ramon, Martinez, Alfredo, Rodriguez, Maria, Ingram, L. O., Preston, J. F., Shanmugam, K. T.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2001
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC95196/
https://ncbi.nlm.nih.gov/pubmed/11325924
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.10.2979-2988.2001
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