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Quantitation of the Capacity of the Secretion Apparatus and Requirement for PrsA in Growth and Secretion of α-Amylase in Bacillus subtilis

Regulated expression of AmyQ α-amylase of Bacillus amyloliquefaciens was used to examine the capacity of the protein secretion apparatus of B. subtilis. One B. subtilis cell was found to secrete maximally 10 fg of AmyQ per h. The signal peptidase SipT limits the rate of processing of the signal pept...

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Udgivet i:J Bacteriol
Principais autores: Vitikainen, Marika, Pummi, Tiina, Airaksinen, Ulla, Wahlström, Eva, Wu, Hongyan, Sarvas, Matti, Kontinen, Vesa P.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2001
Fag:
Genetics and Molecular Biology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC95082/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11222585/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.183.6.1881-1890.2001
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