Preferential Cleavage of Degradative Intermediates of rpsT mRNA by the Escherichia coli RNA Degradosome

RNase E, the principal RNase capable of initiating mRNA decay, preferentially attacks 5′-monophosphorylated over 5′-triphosphorylated substrates. Site-specific cleavage in vitro of the rpsT mRNA by RNase H directed by chimeric 2′-O-methyl oligonucleotides was employed to create truncated RNAs which...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Spickler, Catherine, Stronge, Victoria, Mackie, George A.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Genetics and Molecular Biology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC94981/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11208812/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.183.3.1106-1109.2001
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