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High-Density Microarray-Mediated Gene Expression Profiling of Escherichia coli

A nearly complete collection of 4,290 Escherichia coli open reading frames was amplified and arrayed in high density on glass slides. To exploit this reagent, conditions for RNA isolation from E. coli cells, cDNA production with attendant fluorescent dye incorporation, DNA-DNA hybridization, and hyb...

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Bibliografiske detaljer
Main Authors: Wei, Yan, Lee, Jian-Ming, Richmond, Craig, Blattner, Frederick R., Rafalski, J. Antoni, LaRossa, Robert A.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2001
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC94910/
https://ncbi.nlm.nih.gov/pubmed/11133948
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.2.545-556.2001
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