High-Density Microarray-Mediated Gene Expression Profiling of Escherichia coli

A nearly complete collection of 4,290 Escherichia coli open reading frames was amplified and arrayed in high density on glass slides. To exploit this reagent, conditions for RNA isolation from E. coli cells, cDNA production with attendant fluorescent dye incorporation, DNA-DNA hybridization, and hyb...

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Main Authors: Wei, Yan, Lee, Jian-Ming, Richmond, Craig, Blattner, Frederick R., Rafalski, J. Antoni, LaRossa, Robert A.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2001
Teme:
Physiology and Metabolism
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC94910/
https://ncbi.nlm.nih.gov/pubmed/11133948
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.2.545-556.2001
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