Renaturation of Bacillus thermoglucosidasius HrcA Repressor by DNA and Thermostability of the HrcA-DNA Complex In Vitro

HrcA, a negative control repressor for chaperone expression from the obligate thermophile Bacillus thermoglucosidasius KP1006, was purified in a His-tagged form in the presence of 6 M urea but hardly renatured to an intact state due to extreme insolubility. Renaturation trials revealed that the addi...

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Pubblicato in:	J Bacteriol
Autori principali:	Watanabe, Kunihiko, Yamamoto, Takeshi, Suzuki, Yuzuru
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2001
Soggetti:	Genetics and Molecular Biology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC94861/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11114912/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.183.1.155-161.2001
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