Renaturation of Bacillus thermoglucosidasius HrcA Repressor by DNA and Thermostability of the HrcA-DNA Complex In Vitro

HrcA, a negative control repressor for chaperone expression from the obligate thermophile Bacillus thermoglucosidasius KP1006, was purified in a His-tagged form in the presence of 6 M urea but hardly renatured to an intact state due to extreme insolubility. Renaturation trials revealed that the addi...

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Hlavní autoři: Watanabe, Kunihiko, Yamamoto, Takeshi, Suzuki, Yuzuru
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2001
Témata:
Genetics and Molecular Biology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC94861/
https://ncbi.nlm.nih.gov/pubmed/11114912
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.183.1.155-161.2001
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