A New Thermoactive Pullulanase from Desulfurococcus mucosus: Cloning, Sequencing, Purification, and Characterization of the Recombinant Enzyme after Expression in Bacillus subtilis

The gene encoding a thermoactive pullulanase from the hyperthermophilic anaerobic archaeon Desulfurococcus mucosus (apuA) was cloned in Escherichia coli and sequenced. apuA from D. mucosus showed 45.4% pairwise amino acid identity with the pullulanase from Thermococcus aggregans and contained the fo...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Duffner, Fiona, Bertoldo, Costanzo, Andersen, Jens T., Wagner, Karen, Antranikian, Garabed
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2000
Assuntos:	Enzymes and Proteins
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC94778/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11053376/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.22.6331-6338.2000
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