Cloning and Expression of a Phloretin Hydrolase Gene from Eubacterium ramulus and Characterization of the Recombinant Enzyme

Phloretin hydrolase catalyzes the hydrolytic C-C cleavage of phloretin to phloroglucinol and 3-(4-hydroxyphenyl)propionic acid during flavonoid degradation in Eubacterium ramulus. The gene encoding the enzyme was cloned by screening a gene library for hydrolase activity. The insert of a clone confer...

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Detalles Bibliográficos

Publicado en:	Appl Environ Microbiol
Autores principales:	Schoefer, Lilian, Braune, Annett, Blaut, Michael
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 2004
Materias:	Enzymology and Protein Engineering
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC522078/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15466559/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.10.6131-6137.2004
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