Expression and Characterization of the Chitin-Binding Domain of Chitinase A1 from Bacillus circulans WL-12

Chitinase A1 from Bacillus circulans WL-12 comprises an N-terminal catalytic domain, two fibronectin type III-like domains, and a C-terminal chitin-binding domain (ChBD). In order to study the biochemical properties and structure of the ChBD, ChBD(ChiA1) was produced in Escherichia coli using a pET...

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Pubblicato in:	J Bacteriol
Autori principali:	Hashimoto, Masayuki, Ikegami, Takahisa, Seino, Shizuka, Ohuchi, Nobuhumi, Fukada, Harumi, Sugiyama, Junji, Shirakawa, Masahiro, Watanabe, Takeshi
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2000
Soggetti:	Enzymes and Proteins
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC94488/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10809681/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.11.3045-3054.2000
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