Expression and Characterization of the Chitin-Binding Domain of Chitinase A1 from Bacillus circulans WL-12

Chitinase A1 from Bacillus circulans WL-12 comprises an N-terminal catalytic domain, two fibronectin type III-like domains, and a C-terminal chitin-binding domain (ChBD). In order to study the biochemical properties and structure of the ChBD, ChBD(ChiA1) was produced in Escherichia coli using a pET...

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Main Authors: Hashimoto, Masayuki, Ikegami, Takahisa, Seino, Shizuka, Ohuchi, Nobuhumi, Fukada, Harumi, Sugiyama, Junji, Shirakawa, Masahiro, Watanabe, Takeshi
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2000
Assuntos:
Enzymes and Proteins
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC94488/
https://ncbi.nlm.nih.gov/pubmed/10809681
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