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Cloning, Expression, and Purification of a Thermostable Nonhomodimeric Restriction Enzyme, BslI

BslI is a thermostable type II restriction endonuclease with interrupted recognition sequence CCNNNNN/NNGG (/, cleavage position). The BslI restriction-modification system from Bacillus species was cloned and expressed in Escherichia coli. The system is encoded by three genes: the 2,739-bp BslI meth...

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Bibliografski detalji
Glavni autori: Hsieh, Pei-chung, Xiao, Jian-ping, O'loane, Diana, Xu, Shuang-yong
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2000
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC94369/
https://ncbi.nlm.nih.gov/pubmed/10648519
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