Escherichia coli DNA Topoisomerase I Copurifies with Tn5 Transposase, and Tn5 Transposase Inhibits Topoisomerase I

Tn5 transposase (Tnp) overproduction is lethal to Escherichia coli. Genetic evidence suggested that this killing involves titration of E. coli topoisomerase I (Topo I). Here, we present biochemical evidence that supports this model. Tn5 Tnp copurifies with Topo I while nonkilling derivatives of Tnp,...

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Détails bibliographiques

Publié dans:	J Bacteriol
Auteurs principaux:	Yigit, Hesna, Reznikoff, William S.
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1999
Sujets:	Plasmids and Transposons
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93775/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10322021/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.10.3185-3192.1999
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