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Escherichia coli DNA Topoisomerase I Copurifies with Tn5 Transposase, and Tn5 Transposase Inhibits Topoisomerase I

Tn5 transposase (Tnp) overproduction is lethal to Escherichia coli. Genetic evidence suggested that this killing involves titration of E. coli topoisomerase I (Topo I). Here, we present biochemical evidence that supports this model. Tn5 Tnp copurifies with Topo I while nonkilling derivatives of Tnp,...

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Autors principals: Yigit, Hesna, Reznikoff, William S.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 1999
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC93775/
https://ncbi.nlm.nih.gov/pubmed/10322021
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