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Molecular Cloning, Sequencing, and Expression of a Novel Multidomain Mannanase Gene from Thermoanaerobacterium polysaccharolyticum

The manA gene of Thermoanaerobacterium polysaccharolyticum was cloned in Escherichia coli. The open reading frame of manA is composed of 3,291 bases and codes for a preprotein of 1,097 amino acids with an estimated molecular mass of 119,627 Da. The start codon is preceded by a strong putative riboso...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Cann, Isaac K. O., Kocherginskaya, Svetlana, King, Michael R., White, Bryan A., Mackie, Roderick I.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1999
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93557/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10049399/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.5.1643-1651.1999
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