Purification, Characterization, and Cloning of a Eubacterial 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase, a Key Enzyme Involved in Biosynthesis of Terpenoids

The eubacterial 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) was purified 3,000-fold from Streptomyces sp. strain CL190 to apparent homogeneity with an overall yield of 2.1%. The purification procedure consisted of (NH(4))(2)SO(4) precipitation, heat treatment and anion ex...

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Библиографические подробности

Опубликовано в::	J Bacteriol
Главные авторы:	Takahashi, Shunji, Kuzuyama, Tomohisa, Seto, Haruo
Формат:	Artigo
Язык:	Inglês
Опубликовано:	American Society for Microbiology (ASM) 1999
Предметы:	Enzymes and Proteins
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93504/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9973353/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.4.1256-1263.1999
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