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Purification, Characterization, and Cloning of a Eubacterial 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase, a Key Enzyme Involved in Biosynthesis of Terpenoids

The eubacterial 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) was purified 3,000-fold from Streptomyces sp. strain CL190 to apparent homogeneity with an overall yield of 2.1%. The purification procedure consisted of (NH(4))(2)SO(4) precipitation, heat treatment and anion ex...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Takahashi, Shunji, Kuzuyama, Tomohisa, Seto, Haruo
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 1999
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC93504/
https://ncbi.nlm.nih.gov/pubmed/9973353
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