Cultivation-Independent, Semiautomatic Determination of Absolute Bacterial Cell Numbers in Environmental Samples by Fluorescence In Situ Hybridization

Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes has found widespread application for analyzing the composition of microbial communities in complex environmental samples. Although bacteria can quickly be detected by FISH, a reliable method to determine absolute num...

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Detalhes bibliográficos
Main Authors: Daims, Holger, Ramsing, Niels B., Schleifer, Karl-Heinz, Wagner, Michael
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC93375/
https://ncbi.nlm.nih.gov/pubmed/11722938
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.12.5810-5818.2001
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