Development of a Combined Selection and Enrichment PCR Procedure for Clostridium botulinum Types B, E, and F and Its Use To Determine Prevalence in Fecal Samples from Slaughtered Pigs

A specific and sensitive combined selection and enrichment PCR procedure was developed for the detection of Clostridium botulinum types B, E, and F in fecal samples from slaughtered pigs. Two enrichment PCR assays, using the DNA polymerase rTth, were constructed. One assay was specific for the type...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Dahlenborg, Maria, Borch, Elisabeth, Rådström, Peter
التنسيق: Artigo
اللغة:Inglês
منشور في: American Society for Microbiology 2001
الموضوعات:
Methods
الوصول للمادة أونلاين:https://ncbi.nlm.nih.gov/pmc/articles/PMC93232/
https://ncbi.nlm.nih.gov/pubmed/11571185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.10.4781-4788.2001
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