Combination of Competitive Quantitative PCR and Constant-Denaturant Capillary Electrophoresis for High-Resolution Detection and Enumeration of Microbial Cells

A novel quantitative PCR (QPCR) approach, which combines competitive PCR with constant-denaturant capillary electrophoresis (CDCE), was adapted for enumerating microbial cells in environmental samples using the marine nanoflagellate Cafeteria roenbergensis as a model organism. Competitive PCR has be...

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Bibliographic Details

Published in:	Appl Environ Microbiol
Main Authors:	Lim, Eelin L., Tomita, Aoy V., Thilly, William G., Polz, Martin F.
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 2001
Subjects:	Methods
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93107/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11525983/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.9.3897-3903.2001
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