Combination of Competitive Quantitative PCR and Constant-Denaturant Capillary Electrophoresis for High-Resolution Detection and Enumeration of Microbial Cells

A novel quantitative PCR (QPCR) approach, which combines competitive PCR with constant-denaturant capillary electrophoresis (CDCE), was adapted for enumerating microbial cells in environmental samples using the marine nanoflagellate Cafeteria roenbergensis as a model organism. Competitive PCR has be...

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Detalhes bibliográficos
Main Authors: Lim, Eelin L., Tomita, Aoy V., Thilly, William G., Polz, Martin F.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC93107/
https://ncbi.nlm.nih.gov/pubmed/11525983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.9.3897-3903.2001
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