Quantitation of Viral DNA by Real-Time PCR Applying Duplex Amplification, Internal Standardization, and Two-Color Fluorescence Detection

A real-time PCR method was developed to quantitate viral DNA that includes duplex amplification, internal standardization, and two-color fluorescence detection without the need to generate an external standardization curve. Applied to human parvovirus B19 DNA, the linear range was from 10(2) to at l...

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Detalhes bibliográficos
Main Authors: Gruber, Franz, Falkner, Falko G., Dorner, Friedrich, Hämmerle, Thomas
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2001
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC92947/
https://ncbi.nlm.nih.gov/pubmed/11375203
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.6.2837-2839.2001
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