Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence

BACKGROUND: In real-time PCR, it is necessary to consider the efficiency of amplification (EA) of amplicons in order to determine initial target levels properly. EAs can be deduced from standard curves, but these involve extra effort and cost and may yield invalid EAs. Alternatively, EA can be extra...

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Bibliografski detalji
Glavni autori: Batsch, Anke, Noetel, Andrea, Fork, Christian, Urban, Anita, Lazic, Daliborka, Lucas, Tina, Pietsch, Julia, Lazar, Andreas, Schömig, Edgar, Gründemann, Dirk
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2008
Teme:
Methodology Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2276494/
https://ncbi.nlm.nih.gov/pubmed/18267040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-9-95
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