Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence

BACKGROUND: In real-time PCR, it is necessary to consider the efficiency of amplification (EA) of amplicons in order to determine initial target levels properly. EAs can be deduced from standard curves, but these involve extra effort and cost and may yield invalid EAs. Alternatively, EA can be extra...

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Autors principals: Batsch, Anke, Noetel, Andrea, Fork, Christian, Urban, Anita, Lazic, Daliborka, Lucas, Tina, Pietsch, Julia, Lazar, Andreas, Schömig, Edgar, Gründemann, Dirk
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2008
Matèries:
Methodology Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2276494/
https://ncbi.nlm.nih.gov/pubmed/18267040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-9-95
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