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Chromosomal Gene Inactivation in the Green Sulfur Bacterium Chlorobium tepidum by Natural Transformation

Conditions for inactivating chromosomal genes of Chlorobium tepidum by natural transformation and homologous recombination were established. As a model, mutants unable to perform nitrogen fixation were constructed by interrupting nifD with various antibiotic resistance markers. Growth of wild-type C...

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Hlavní autoři: Frigaard, Niels-Ulrik, Bryant, Donald A.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2001
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC92905/
https://ncbi.nlm.nih.gov/pubmed/11375161
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.6.2538-2544.2001
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