Chromosomal Gene Inactivation in the Green Sulfur Bacterium Chlorobium tepidum by Natural Transformation

Conditions for inactivating chromosomal genes of Chlorobium tepidum by natural transformation and homologous recombination were established. As a model, mutants unable to perform nitrogen fixation were constructed by interrupting nifD with various antibiotic resistance markers. Growth of wild-type C...

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Bibliographic Details

Published in:	Appl Environ Microbiol
Main Authors:	Frigaard, Niels-Ulrik, Bryant, Donald A.
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 2001
Subjects:	Genetics and Molecular Biology
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92905/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11375161/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.6.2538-2544.2001
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