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Development and Validation of Corynebacterium DNA Microarrays

We have developed DNA microarray techniques for studying Corynebacterium glutamicum. A set of 52 C. glutamicum genes encoding enzymes from primary metabolism was amplified by PCR and printed in triplicate onto glass slides. Total RNA was extracted from cells harvested during the exponential-growth a...

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Publicado no:Appl Environ Microbiol
Principais autores: Loos, Andrea, Glanemann, Christoph, Willis, Laura B., O'Brien, Xian M., Lessard, Philip A., Gerstmeir, Robert, Guillouet, Stéphane, Sinskey, Anthony J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2001
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92872/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11319117/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.5.2310-2318.2001
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