Development and Validation of Corynebacterium DNA Microarrays

We have developed DNA microarray techniques for studying Corynebacterium glutamicum. A set of 52 C. glutamicum genes encoding enzymes from primary metabolism was amplified by PCR and printed in triplicate onto glass slides. Total RNA was extracted from cells harvested during the exponential-growth a...

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Autors principals: Loos, Andrea, Glanemann, Christoph, Willis, Laura B., O'Brien, Xian M., Lessard, Philip A., Gerstmeir, Robert, Guillouet, Stéphane, Sinskey, Anthony J.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2001
Matèries:
Methods
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC92872/
https://ncbi.nlm.nih.gov/pubmed/11319117
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.5.2310-2318.2001
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