In Situ Accessibility of Escherichia coli 23S rRNA to Fluorescently Labeled Oligonucleotide Probes

One of the main causes of failure of fluorescence in situ hybridization with rRNA-targeted oligonucleotides, besides low cellular ribosome content and impermeability of cell walls, is the inaccessibility of probe target sites due to higher-order structure of the ribosome. Analogous to a study on the...

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Hauptverfasser: Fuchs, Bernhard M., Syutsubo, Kazuaki, Ludwig, Wolfgang, Amann, Rudolf
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2001
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Methods
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC92673/
https://ncbi.nlm.nih.gov/pubmed/11157269
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.2.961-968.2001
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