Magnetic Bead Capture Eliminates PCR Inhibitors in Samples Collected from the Airborne Environment, Permitting Detection of Pneumocystis carinii DNA

PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis c...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Maher, Nancy, Dillon, H. Kenneth, Vermund, Sten H., Unnasch, Thomas R.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Public Health Microbiology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92598/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11133478/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.1.449-452.2001
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