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Magnetic Bead Capture Eliminates PCR Inhibitors in Samples Collected from the Airborne Environment, Permitting Detection of Pneumocystis carinii DNA

PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis c...

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Bibliografiset tiedot
Päätekijät: Maher, Nancy, Dillon, H. Kenneth, Vermund, Sten H., Unnasch, Thomas R.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: American Society for Microbiology 2001
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC92598/
https://ncbi.nlm.nih.gov/pubmed/11133478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.1.449-452.2001
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