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Magnetic Bead Capture Eliminates PCR Inhibitors in Samples Collected from the Airborne Environment, Permitting Detection of Pneumocystis carinii DNA

PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis c...

詳細記述

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書誌詳細
主要な著者: Maher, Nancy, Dillon, H. Kenneth, Vermund, Sten H., Unnasch, Thomas R.
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology 2001
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC92598/
https://ncbi.nlm.nih.gov/pubmed/11133478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.1.449-452.2001
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