Magnetic Bead Capture Eliminates PCR Inhibitors in Samples Collected from the Airborne Environment, Permitting Detection of Pneumocystis carinii DNA

PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis c...

Full description

Saved in:
Bibliographic Details
Main Authors: Maher, Nancy, Dillon, H. Kenneth, Vermund, Sten H., Unnasch, Thomas R.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2001
Subjects:
Public Health Microbiology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC92598/
https://ncbi.nlm.nih.gov/pubmed/11133478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.1.449-452.2001
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items