Preparing a human membrane and secreted protein-enriched cDNA library using PCR primers derived from a genomic database

We describe here a strategy for preparing a human membrane and secreted protein (MSP)-enriched cDNA library based on human MSP- and non-MSP-encoding cDNA sequences in the databases. The signal peptide parts of the MSP-encoding cDNA sequences, which currently comprise about half of the estimated tota...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Fan, Yi, Wu, Chih Yuan, Chen, Cheng Wei, Chang, Tse Wen, Lim, Carmay
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 2001
Témata:	NAR Methods Online
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92583/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11713334/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.22.e114
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