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Preparing a human membrane and secreted protein-enriched cDNA library using PCR primers derived from a genomic database

We describe here a strategy for preparing a human membrane and secreted protein (MSP)-enriched cDNA library based on human MSP- and non-MSP-encoding cDNA sequences in the databases. The signal peptide parts of the MSP-encoding cDNA sequences, which currently comprise about half of the estimated tota...

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Detalhes bibliográficos
Main Authors: Fan, Yi, Wu, Chih Yuan, Chen, Cheng Wei, Chang, Tse Wen, Lim, Carmay
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC92583/
https://ncbi.nlm.nih.gov/pubmed/11713334
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