Introduction of an N-Glycosylation Site Increases Secretion of Heterologous Proteins in Yeasts

Saccharomyces cerevisiae is often used to produce heterologous proteins that are preferentially secreted to increase economic feasibility. We used N-glycosylation as a tool to enhance protein secretion. Secretion of cutinase, a lipase, and llama V(HH) antibody fragments by S. cerevisiae or Pichia pa...

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Detalhes bibliográficos
Main Authors: Sagt, C. M. J., Kleizen, B., Verwaal, R., de Jong, M. D. M., Müller, W. H., Smits, A., Visser, C., Boonstra, J., Verkleij, A. J., Verrips, C. T.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2000
Assuntos:
Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC92403/
https://ncbi.nlm.nih.gov/pubmed/11055947
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