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Quantitative Analysis of Small-Subunit rRNA Genes in Mixed Microbial Populations via 5′-Nuclease Assays

Few techniques are currently available for quantifying specific prokaryotic taxa in environmental samples. Quantification of specific genotypes has relied mainly on oligonucleotide hybridization to extracted rRNA or intact rRNA in whole cells. However, low abundance and cellular rRNA content limit t...

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Publicado no:Appl Environ Microbiol
Principais autores: Suzuki, Marcelino T., Taylor, Lance T., DeLong, Edward F.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2000
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92356/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11055900/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.11.4605-4614.2000
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