Quantitative Analysis of Small-Subunit rRNA Genes in Mixed Microbial Populations via 5′-Nuclease Assays

Few techniques are currently available for quantifying specific prokaryotic taxa in environmental samples. Quantification of specific genotypes has relied mainly on oligonucleotide hybridization to extracted rRNA or intact rRNA in whole cells. However, low abundance and cellular rRNA content limit t...

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Détails bibliographiques

Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Suzuki, Marcelino T., Taylor, Lance T., DeLong, Edward F.
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 2000
Sujets:	Methods
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92356/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11055900/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.11.4605-4614.2000
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