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A Bead-Based Method for Multiplexed Identification and Quantitation of DNA Sequences Using Flow Cytometry

A new multiplexed, bead-based method which utilizes nucleic acid hybridizations on the surface of microscopic polystyrene spheres to identify specific sequences in heterogeneous mixtures of DNA sequences is described. The method consists of three elements: beads (5.6-μm diameter) with oligomer captu...

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Detalhes bibliográficos
Main Authors: Spiro, Alexander, Lowe, Mary, Brown, Drew
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC92294/
https://ncbi.nlm.nih.gov/pubmed/11010868
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