Hydrogen Peroxide Sensitivity of Catechol-2,3-Dioxygenase: a Cautionary Note on Use of xylE Reporter Fusions under Aerobic Conditions

Catechol-2,3-dioxygenase (C23O) of Pseudomonas putida, encoded by the xylE gene, was found to be sensitive to hydrogen peroxide (H(2)O(2)) when used as a reporter in gene fusion constructs. Exposure of Pseudomonas aeruginosa katA or katA katB mutants harboring katA- or katB-lacZ (encoding β-galactos...

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Main Authors: Hassett, Daniel J., Ochsner, Urs A., Groce, Stephanie L., Parvatiyar, Kislay, Ma, Ju-Fang, Lipscomb, John D.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2000
Assuntos:
Enzymology and Protein Engineering
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC92268/
https://ncbi.nlm.nih.gov/pubmed/10966438
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