Autocloning and Amplification of LIP2 in Yarrowia lipolytica

We synthesized a Yarrowia lipolytica strain overproducing lipase for industrial applications by using long terminal repeat (ζ) of the Y. lipolytica retrotransposon Ylt1 and an allele of URA3 with a promoter deletion to construct JMP3. JMP3 is a derivative of plasmid pHSS6 carrying a NotI-NotI casset...

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Bibliografiske detaljer
Main Authors: Pignède, Georges, Wang, Hui-Jie, Fudalej, Franck, Seman, Michel, Gaillardin, Claude, Nicaud, Jean-Marc
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2000
Fag:
Genetics and Molecular Biology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC92146/
https://ncbi.nlm.nih.gov/pubmed/10919782
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