Introduction of Raw Starch-Binding Domains into Bacillus subtilis α-Amylase by Fusion with the Starch-Binding Domain of Bacillus Cyclomaltodextrin Glucanotransferase

We constructed two types of chimeric enzymes, Ch1 Amy and Ch2 Amy. Ch1 Amy consisted of a catalytic domain of Bacillus subtilis X-23 α-amylase (Ba-S) and the raw starch-binding domain (domain E) of Bacillus A2-5a cyclomaltodextrin glucanotransferase (A2-5a CGT). Ch2 Amy consisted of Ba-S and D (func...

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Autores principales: Ohdan, Kohji, Kuriki, Takashi, Takata, Hiroki, Kaneko, Hiroki, Okada, Shigetaka
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2000
Materias:
Enzymology and Protein Engineering
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC92111/
https://ncbi.nlm.nih.gov/pubmed/10877806
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