Introduction of Raw Starch-Binding Domains into Bacillus subtilis α-Amylase by Fusion with the Starch-Binding Domain of Bacillus Cyclomaltodextrin Glucanotransferase

We constructed two types of chimeric enzymes, Ch1 Amy and Ch2 Amy. Ch1 Amy consisted of a catalytic domain of Bacillus subtilis X-23 α-amylase (Ba-S) and the raw starch-binding domain (domain E) of Bacillus A2-5a cyclomaltodextrin glucanotransferase (A2-5a CGT). Ch2 Amy consisted of Ba-S and D (func...

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Опубликовано в::	Appl Environ Microbiol
Главные авторы:	Ohdan, Kohji, Kuriki, Takashi, Takata, Hiroki, Kaneko, Hiroki, Okada, Shigetaka
Формат:	Artigo
Язык:	Inglês
Опубликовано:	American Society for Microbiology (ASM) 2000
Предметы:	Enzymology and Protein Engineering
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92111/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10877806/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.7.3058-3064.2000
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